EzWay Annexin V-FITC Apoptosis Detection Kit

EzWay Annexin V-FITC Apoptosis Kit can identify apoptosis at an earlier stage than DNA-based assays such as TUNEL.

This kit is designed for the detection of apoptotic cells using flow cytometry. This kit contains Annexin V-FITC, a fluorescent dye that binds to phosphatidylserine on the surface of apoptotic cells, and propidium iodide for the identification of necrotic cells. The kit is suitable for both adherent and suspension cells, providing a reliable and convenient method for apoptosis analysis. With highly sensitive and specific reagents, this kit allows for the accurate quantification of apoptotic cell populations in biological samples. Overall, the Annexin V-FITC Apoptosis Kit is an essential tool for researchers studying cell death and apoptosis in fields such as cancer biology and drug development.

 

Assay Principle

Annexin V is a calcium-dependant phospholipid binding protein that has a high affinity for phosphatidylserine (PS), a plasma membrane phsopholipid. In non-apoptotic cells, most PS are localized at the inner layer of the plasma membrane, but after inducing apoptosis, PS are exposed to the extracellular environment, and then Annexin V binds to PS.

Cells that stain positive for Annexin V-FITC (green) and negative for PI are undergoing apoptosis. Cells that stain positive for both Annexin V-FITC (green) and PI (red) are either in the end stage of apoptosis, are undergoing necrosis, or are already dead.

Cells that stain negative for both Annexin V-FITC and PI are alive and not undergoing measurable apoptosis.

 

Features

• Utilizes FITC-conjugated Annexin V protein for detection of cells undergoing apoptosis
• Annexin V FITC binds to the membranes of apoptotic cells
• Detection by flow cytometry or fluorescence microscopy

 

Kit Components

• Annexin V-FITC
• 5X Binding buffer
• Propidium Iodide (PI)